NASC

NASC ID: N849356

Name: WiscDsLox233237_16F

ABRC stock number: CS849356

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox233237_16F .

Wiscseq_DsLox233237_16F.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G62130.1 and AT1G62150.1-UTR3 (at chr 1 pos 22969005 (C/22969005-22969036) on the TAIR10). The sequences' 1-32 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 0, R 3, C 4 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452980.

Wiscseq_DsLox233237_16F.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G39851.1-UTR3 and AT2G39855.1-P355 (at chr 2 pos 16635537 (C/16635537-16635601) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 85-100 bps onto the T-DNA. Pools P 0, R 3, C 4 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453285.

Donation Date: 2006-09-04

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox233237_16F

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox233237_16F	WiscDsLox233237_16F	AT1G62150 AT2G39851 AT2G39855 At1g62130